Alfalfa is one most important crops in the Hamedan area and native to this area. Different arthropods were found in this ecosystem. Up to now more than 200 arthropods were collected and identified associated with alfalfa in phytophagous, predacious, pollination, parasitoids and saprophagous roles in the Hamedan area, of which the Araneae subclass is predacious and prey on different insects and mites in this ecosystems. Currently less is known of the spider fauna in this area. This study was conducted to determine spiders associated with alfalfa in the Hamedan vicinity, 2013. In order 10 families, 19 genera and 20 species of this area were collected and identified, and their scientific names as follows: Agelenidae: Agelescape gideoniLevy; Araneidae: Aculepeirasp., Argiope aurantiaLucas; Theridiidae: Steatoda paykul-liana(Walckenaer), Episinus truncatusLatreille; Pisauridae: Pisaura mirabilis(Clerck); Oxyopi-dae: Oxyopes iranicusEsyunin, Oxyopes lineatusLatreille; Linyphiidae: Microlinyphia pusilla (Sundevall), Philodromidae: Thanatus vulgarisSimon; Pholcidae: Pholcus alticepsSpassky; Gnaphosidae: Drassodes caspiusPonomarev & Tsvetkov, Drassodes charitonoviTuneva, Micariasp., Nomisia recepta(Pavesi), Zelotes longipes(Koch); Thomisidae: Thomisussp., Xysticus ninniiThorell, Xysticus striatipesKoch; Lycosidae: Pardosa agricola(Thorell), Pardosa proxima (Koch), Hogna radiata(Latreille, 1817), Hogna radiata(Latreille, 1817). Among them P. agricola, T. vulgaris, O. iranicus, S. paykullianawere found abundantly and with a wide distribution.
