Mites associated with gladiolus were collected and identified within 2011-2013. Among the collected mites some were in phytophagous role which including: Tetranychus urticae Koch, Rhizoglyphus echinopus (Fumouze & Robin) and some others in predatory role like: Neoseiulus marginatus (Wainstein), Neoseiulus sugonjaevi (Wainstein & Abbasova) ,Hypoaspis aculeifer (Gaeolaelaps) (Canestrini), Hypoaspis queenslandica (Gaeolaelaps) (Womersley), Neophyllobius persianeensis Khanjani & Ueckermann, Raphignathus gracilis (Rack), Neognathus terrestris Summers & Schlinger, Eustimaeus nasrinae Khanjani & Ueckermann, Hoplocheylus fereshtehae Amin, Khanjani & Ueckermann 2014, Hoplocheylus. sp., Curteria episcopalis Koch. Among the predatory mites in soil Hypoaspis aculeifer (was dominat species and has been the one of the major predators in the biological control of pests in greenhouses. Currently H.miles and H.aculiefer are known as biological agentes. They are effective in control of insects, mites, nematodes and fungus in the soil
