Due to their nutritional diversity, frequency and being able to live everywhere, mites could be considered as good representative of existing arthropods in soil. They play vital role in balancing and refining soil ecosystems, especially in absorbing and storing heavy metals such as cadmium as well as pesticides. In fact, their frequency and diversity can be the indices of ecosystem health. Consequently, frequency and biodiversity of prostigmata mites in some regions of Hamedan, Kurdistan and Kermanshah provinces over the years 2012-2014 were investigated. Soil samples and aboveground plant parts were carried to lab and using Berlease Funnel and isolated shaking method were respectively and directly placed in Hoyer’s medium. In total, 45 species belong to 20 genera from 12 families were collected and identified and 31 species were predatory role. Utilizing Brillouin, Simpson, and Shannon- Wiener indices, it was proved that Kurdistan province is richer in terms of species. Among herbivorous, Eriophyes tristriatus species had the highest relative abundance, 14.2%, predatory mites, Raphignathus protaspis، Spinibdella cronini and Raphignathus hecmatanaensis with 13%, 12.5% and 8.2% had the highest frequencies, respectively.
