A faunistic study of prostigmatic mites (Acari: Prostimata) associated with olive orchards in Guilan province, Northern Iran was carried out during 2012-2013. Soil and plant foliage samples were collected from olive orchards of Guilan Province. Mites were ex-tracted from soil and plant foliage by placing them on Berlese funnel or direct examina-tion of leaves under a stereomicroscope. Collected mites were cleared in Lactophenol and Nesbitt’s fluids and mounted in Hoyer’s medium on microscopic slides. Totally, 24 species from 18 genera and 13 families were collected and identified. Among them 23 species belonging to 17 genera are new for olive orchards mites fauna of Iran. A tabula-ted checklist for prostigmstic mites associated with olive trees in Iran is also provided.
