The myelin figure (MF) is one of the basic structures of lipids, and the study of their formation and the effect of various parameters on their growth is useful in understanding several biological processes. In this paper, we address the influence of the pH degree of the surrounding medium on MF dynamics. We introduce a tunable shearing digital holographic microscopy arrangement to obtain quantitative and volumetric information about the complex growth of MFs. Our results show that (1) the time evolution of relative length and volume changes of MFs follows a power-law, (2) the acidity facilitates the growth rate, and (3) the acidic environment causes the formation of thicker MFs. © 2
