Left–right differential erector spinae (ES) muscle strengthening is required to correct ES muscle imbalances. The objective was to test the effect of 6 body positions on the differential activation of the ES muscles. In 14 able-bodied young women, using a surface electromyography system, the bilateral ES muscles activity at the third lumbar (ESL3) and the 10th (EST10) and 6th (EST6) thoracic vertebral levels was measured with the contralateral arm and leg lifted in the prone and quadruped conditions and with a single arm lifted in the quadruped position. Results showed that the activity of the ESL3 was symmetrical (P > .05) and significantly smaller than that of the thoracic ES muscles in all body positions (P < .01). The EST10 and EST6 were differentially activated in all tests (P < .001). Besides, the differential activation was higher in the contralateral-arm and -leg lift in the quadruped position than in the other positions. In conclusion, contralateral-arm and -leg lift and single-arm lift in the quadruped and prone positions are capable of differentially activating the ES muscles on one side more than the other side. Further studies are recommended to examine the effectiveness of these exercises on the correction of ES muscle imbalances in clinical populations.