Influence of sodium dodecylsulfate (SDS) and magnetite nanoparticles (NPs), simultaneosly, on the interfacial tension of n-hexane–water is investigated at different temperatures. The results show SDS, by itself, has positive adsorption, however, negative adsorption corresponds to just NPs. Furthermore, the interfacial tension decreases with temperature, because of intermolecular attenuation. It is while, by introducing SDS into different dosage of NPs, interfacial tension is declined significantly, because of attaching SDS to positivley charged NPs, and therfore, diminishing electrostatic repulsive interactions between adsorbed SDS molecules.