The effects of inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi Glomus intraradices and non-inoculation on root colonization, plant growth, agronomic characteristics, essential oil content and essential oil yield in Pimpinella anisum, were studied under field conditions. The experiment was carried out in a field conditions, in randomized complete block design with three replications. The VAM inoculation significantly increased the root colonization, number of umbel per plant, number of seeds per umbelet, seed yield, essential oil content and essential oil yield as compared to non-inoculated treatment. The effect of VAM inoculation on the root colonization, growth and seed yield of Pimpinella anisum was more pronounced, indicating Pimpinella anisum as a highly mycorrhizal dependent genotype. VAM inoculation most markedly increases the uptake of phosphorus. In fact; the VAM inoculation could significantly increase root colonization, growth, and essential oil yield in Pimpinella anisum for obtaining economic production under field conditions.