Abstract background: Leishmaniasis is an extensive lesion. Laboratory studies in this field could help improve diagnosis and treatment. This study focuses on pro-inflammatory gene expression in mice infected with Leishmania wound and infected mice without wound. Methods: The specific primers were designed for four pro-inflammatory genes including IL-1α, IL-1β, IL-12P35, IL-12P40, TNF- α and IFN- γ. Expression of these genes was analyzed using by RT-PCR in the wound and spleen in Leishmania infected with and without wound in BALB/c mice. Results: Pro-inflammatory genes were expressed in mice infected with the wound contains (TNF-α in spleen & TNF- α and IL-1β in wound) and in infected mice without wound contains (TNF-α in spleen & IL-1β in the injection position). Conclusion: Study on inflammatory gene expression in vitro model of leishmaniasis wounds can help improving this complication in vitro and vivo studies and is important for diagnostic strategies.
