Background and Aim: Colon cancer is known to be the third most common cancer and the fourth leading cause of death in the world. The organic chemistry research team of Bu-Ali Sina University has synthesised Flutamide with a sulfonamide functional group which can improve the effect of the existing drug. In this study, the survival rate of mouse colon carcinoma cell line (CT26) treated with flutamide and modified flutamide is evaluated. Methods: Cancer cells survival were determined using the MTT assay. CT26 cells cultured in 96-well plates and incubated with the drugs in concentrations of 20, 40, 60, 80, and 100μM for 24 hours in 37°C and 5% CO2. Results: The IC50 of the Flutamide was 2.87 μM and that of the modified drug was 35.27 μM after 24 hours of incubation. The anticancer effect of the modified Flutamide drug was lower than that of existing drug. Conclusion: The Flutamide drug has an acceptable anticancer effect against colon carcinoma, while the addition of the functional sulfonamide group has reduced the anticancer effect of the Flutamide. One of the reasons for this reduction may be the chemical and spatial change of the reason for adding this group is the factor that has affected the biochemical activity of this drug.
