حسین رضوان

Introduction Leishmania is an intracellular pathogen that affects dendritic cells and macrophages. The various clinical forms of this parasite include: visceral leishmaniasis(kala-azar), cutaneous(CL) and mucocutaneous lesions. This study was to evaluate macrophage phagocytosis CL models that have been treated with mesenchymal stem cells. Materials and Methods A total number of 60 female Balb/C mice aged 6 to 8 weeks old with weight average of 29- 35g were purchased from pasteur institute(Tehran,Iran).Treatment group received MSCs(Intralesion 1×106/100ul). Peritoneal macrophages were collected by aspiration cold physiology serum(with pen/strep 5%). Cells treated with NBT solution(1mg/ml) after incubation, optical absorbance of trial measured(450nm). Result The amount of phagocytosis in the MSC treatment group with a treatment duration of 30 days showed a significant increase compared to the control and standard treatment groups p˂0.05. Conclusion Considering the key role of macrophage in the treatment of cutaneous leishmaniasis, increasing the amount of phagocytosis can be important following treatment with mesenchymal cells.