A number of binuclear and mononuclear macroacyclic Schiff base complexes containing piperazine moieties were synthesized. The binuclear complexes (M2L1) characterized by elemental analysis, IR and, also Ni(II) complex by a X-ray single crystal structural analysis. In this case, X-ray showed that each nickel atom is in a mer- N3O3 octahedral coordination environment. Moreover, mononuclear macroacyclic Schiff base complexes (ML2 and ML3) were prepared based on the condensation reaction of an amine containing piperazine moiety and 2-hydroxy banzaldehyde or formyl pyridine in 1:2 mol ratio in the presence of Ni(II), Cu(II), and Co(II) ions. All of compounds were characterized by elemental analyses, FT-IR, mass spectrometry and ligands characterized by 1H and 13C NMR spectroscopy. The cytotoxicity of the complexes was evaluated against two different cancer cell lines including MCF-7 (breast) and A549 (lung) adenocarcinoma cells. In general, binuclear complexes, possesses a higher cytotoxic effect against the tested cells than the other complexes. In addition, the biological assessment of complexes have been examined via molecular docking.
