Mushrooms contain a variety of secondary metabolites. including various phenolic compounds and ergotioneine. which have been known excellent antioxidant. The present study reports the comparison of antioxidant properties, total phenolic, ergothioneine and mineral contents of the most consumed strains of Agaricus bisporus (white or brown colors). Rhe samples were fruiting body. mycelia produced at farm( old mycelium) and in vitro (young mycelium). The mushroom strain with the highest ergothioneine phenolic compounds and antioxidant potential was the strain A-SPA15 (brown color). There were variations in mineral content of A. bisporus harvested from the fruiting body, old mycelium and young mycelium. This study contributes to the data ralative ro A. bisporus consumed as fresh mushrooms and the possibility of in vitro production as a source of bioactive compounds.