In July 2014, symptoms of berry cracking and blue mould were observed on table grapes grown in a grapevine arboretum in Hamedan Province, Iran. Symptoms began as small, round, water-soaked lesions, and over 4 to 6 days enlarged gradually in diameter, developed into soft, blue mycelium near the centre of infection sites. Fungal strains were isolated from the symptomatic tissues. Based on morphological characteristics, sequence analysis of β-tubulin gene and a pathogenicity test, the fungal strains were identified as Penicillium sumatrense. This is thought to be the first report of P. sumatrense as a blue mould on grape in Iran.