In this work, Graphene oxide (GO)was synthesis by graphite [1]. Compared with deposition methods, Electrophoretic deposition (EPD) has attracted a lot of attention and the basis of this is that this method is cost-effective and easy way to deposition. GO is deposited on stainless steel mesh by anodic EPD because of negative charge of GO. The EPD's factors were optimized with regard to deposited mass. The EPD process was carried out under a DC voltage of 30 V and the deposition time of 20 min with constant concentration (3mg/ml). The mesh-GO placed in vacuum oven in different temperature to investigate thermal annealing. Morphology of the samples was characterized via scanning electron microscopy (SEM) analysis. Energy-dispersive X-ray (EDX) and FT-IR analyses confirm the reduction of GO with thermal annealing. Here in, water contact angle (WCA) measurement was used to study wettability of surface of annealed samples. Increasing thermal annealing temperature leads to decreasing wettability, WCA results show that the superhydrophilic steel surface is changed to superhydrophobic after deposition of GO and then thermal annealing at 200 ºC for 2h. On top of that influence of thermal annealing of GO on cyclic voltammetry (CV) and charge/discharge time are investigated. The electrochemical process was performedunder concentration of electrolyte solution 1M KOH. The results suggest that oxidation and reduction peaks were declined with increasing temperature of heat treatment. Comparison discharge time of samples show that an obvious decrease in discharge time.