he response of microorganisms in different soils to each of the used antibiotics is not the same. Although the abundance of microorganisms in soils contaminated with heavy metals is less, but their ability to live against antibiotics is higher. Overall, the resistance of bacteria in metal-contaminated soils was higher against antibiotics, and the harmful consequences of antibiotics in the medium and long time, especially in soils contaminated with heavy metals (rangeland and mine) disappeared.