With the increase of environment pollution by heavy metals in the past decades and its effects on the food chain, phytoremediation can be a way to reduce pollution of metals from the environment. This study aiming to evaluate the ability of sunflower inoculated with Glomus mosseae for grow and uptake of lead (Pb) in a soil contaminated with zero, 600, 1200 and 1800 mg kg-1 Pb. This study was carried out in a greenhouse cultivation. Increasing lead contamination decreased root and shoot biomasses of sunflower significantly. In contrast, inoculation with mycorrhizal fungi, Glomus mosseae, increased plant growth in soil contaminated with lead. Percentage of root colonization by Glomus mosseae was rose by increasing the level of Pb to 1200 mg kg-1 and was reduced at 1800 mg kg-1 Pb. Lead concentration in mycorrhizal sunflower varied in the shoot biomass from 26.67 to 166.67 mg kg-1 and in the root biomass from 45.14 to 435.43 mg kg-1 Pb (for zero to 1800 mg kg-1 Pb respectively). Dry weight was varied and increased significantly above the permissible limit (30 mg kg-1 Pb), by increasing the lead level. Translocation and enrichment factors were less than 1 and sunflower stored more Pb in its root rather than its shoot.