Objective- We know pet owners are concern over the issue of euthanasia and how to do it. Therefore to make decision which technique is more appropriate for euthanasia of pets is a highly stressful situation for veterinarians and may be source of some dilemmas; also, there are some matters i.e. financial drawbacks, when common methods of euthanasia such as overdose injection of anesthetic agents are used1, so, in this paper a new humane method for euthanasia was described by intrathecal injection of Lidocaine in dog. Design- Pilot clinical trial Animals- Seven dogs which they suffered were nominated for euthanasia. Procedures- After induction of anesthesia by combination of xylazine-acepromazine and ketamine2, according to Modified Glasgow Coma Scoring System, unconsciousness of all animals was established. Then, 2- 8 mL lidocaine was injected intrathecally, and quality of death, vital signs and electrical activity of heart were assessed subsequently. Results- The results suggest that direct application of lidocaine on medullary region of CNS after induction anesthesia may causes loss of consciousness, respiratory arrest, bradycardia, atrioventricular block and finally cardiac arrest. Conclusion and Clinical Relevance- Results showed that this method can be considered as a humane, affordable, rapid and irreversible way to end the dog’s life where the death is inevitable and there is no kind of facilities for putting to sleep an animal3.