This work describes the development of an easy to fabricate biosensor for determination of oxytetracycline (OTC) in basis of electrocatalytically oxidation of OTC by horse radish peroxidase (HRP) in the presence of H2O2. This work is first report on determination of OTC by enzymatic modified electrode. Developed electrode was fabricated by modifying of CPE with HRP and MWCNTs. This electrode provides sensitive and selective determination for OTC. Detection limit, linear range and sensitivity for OTC determination were completely satisfactory and were about 35 nM, 15 M–1.5 mM and 18.74 A/mM, respectively. Applicability of developed electrode was verified with successfully determination of OTC in pharmaceutical and biologic samples. Also developed electrode was showed the selective determination of OTC in presence of uric and folic acid.
