BACKGROUND AND OBJECTIVES: Innate immunity is the first defense of arthropods. Development of arthropods compared to other organisms, while live near potentially harmful microorganisms. It showed that simple but highly effective congenital immune system in arthropods. In previous studies, the effect of hemolymph of different scorpions has been worked on the growth and proliferation of different bacteria. The aim of this study is investigating the antimicrobial activity of Scorpio maurus kruglovi Hemolymph. MATERIALS AND METHODS: Scorpions of the species S. Maurus kruglovi were collected of state of Kermanshah. In this study hemolymph was collected by a puncture between the second and third segments of the scorpion metasoma. Antibiogram discs are coated with hemolymph, placed on bacterial culture and incubated for 24 h. In this study were used ACT259222(Escherichia coli), ATCC25923(Staphylococcus aureus), ATCC14028(Salmonella typhimurium), ATCC27853(Pseudomonas aeruginosa) and ATCC12022(Shigella flexneri). RESULTS AND DISCUSSION: Obtained results show that hemolymph of this scorpion wasn’t affect on the proliferation of both Gram-positive and Gram-negative bacteria. In this study hemolymph of Scorpio maurus kruglovi unlike another previous research hasn’t bactericidal power effects.
