AIM: Apple as a main temperate zone fruit is considered as a nutritive crop. Some factors like cultivar and climate may influence on fruit components and physical properties. In this study physico-chemical characteristic of apple fruit was investigated as a function of cultivars and climate condition. METHODS: In this study some physico-chemical characteristics of two apple cultivars, red delicious and golden delicious in five regions including Damavand, Khorram abad, shiraz, Shah Rood and Tabriz were studied using completely randomized design with 10 treatments and four repetitions, to determine the best location for high quality apple production. RESULTS: According to the results, there were significant differences in all characteristics between the cultivars and also among the region (P<0.01). Red delicious from Damavand had the highest anthocyanin level. Red delicious fruit from Shiraz and Damavand had 6.59 mg/g and 5.76 mg/g malic acid which were the most among the region. the highest level of total phenol was observed in red delicious from Khorram abad (2.01 mg/g and red delicious from shiraz (1.92 mg/g). the highest level of flavonoides was observed in golden delicious from shiraz (1.79 mg/g), followed by red delicious from Shah Rood and golden delicious from Tabriz. the most glucose was in red delicious from Damavand (7.53 mg/100 g), and the most sucrose was in red delicious from Khorram abad (8.2 mg/100 g). red delicious from Damavand had the highest weight (240.8 g) and golden delicious from Tabriz had the least weight (101.27 g).