Background and Aim: Salmonella is a diverse bacterial species comprising over 2500 serotypes. Most of the Salmonella serotypes are part of S. enterica subspecies enterica and the majority of human and animal infections are due to Salmonella serotypes belonged to this subspecies. Among these, enteric infections are very important and may lead to huge economic losses in dairy herds. The objective of the present study was to investigate Salmonella infections in feces obtained from bovine farms located in Hamedan province of Iran. Methods: A total of 70 fecal samples were collected from cattle herds and cultured in Rappaport transport medium. Thereafter, Salmonella isolates were identified based on the culture characteristics on differential media including Xylose-lysine deoxycholate (XLD), Brilliant green agar (BG) medium, Citrate, Triple sugar iron (TSI) agar, SIM and MR-VP. Results: On the basis of culture and biochemical features, 8 (11.42%) out of 70 examined fecal samples were positive for Salmonella. Conclusion: The results showed a relatively high prevalence of Salmonella in examined animals. Therefore, it is suggested to take strict strategies to prevent and control Salmonella infections in this region. Especially as Salmonella are important zoonotic pathogens.