In order to investigate the effect of putrescine and zinc sulfate on black seed plant at three levels of putrescine (zero, 0.25 and 0.5 mM) and zinc sulfate concentration at three levels (zero, 0.05 and 0.1%) in the form of a design Randomized complete blocks were performed with three replications. The investigated characteristics were measured in three groups: growth characteristics, phytochemical characteristics, and oil percentage and quality. The results showed that the simple effects of putrescine and zinc sulfate on plant growth characteristics such as height, root length, number of flowers, centipede weight, fresh and dry weight of shoots and fresh and dry weight of roots were significant at 1% level. However, the mutual effects of putrescine and zinc sulfate were significant only on plant height at the level of five percent and on the number of inflorescences, fresh weight of shoots and fresh and dry weight of roots at the level of one percent. The effect of putrescine and sulfate was significant only on chlorophyll b, and the simple effect of putrescine on total phenol at the level of 1% and the interaction effect of putrescine and zinc sulfate on the content of total phenol, flavonoid at the level of 1% and oil percentage at the level of 5% were significant. And it was not significant on the antioxidant capacity. The analysis of fatty acids showed that the treatments had a significant effect on the percentage of oleic acid and 2-cis-alpha linoleic acid at the level of one percent, and had a significant effect on the percentage of palmitic acid and stearic acid at the level of five percent.
