Separation instruments including gas chromatography, high pressure/performance liquid chromatography, capillary electrophoresis, and ion chromatography have become a part of most of the analytical laboratories. These methods are primarily used for the separation of complex samples and quantitation of a target analyte in the sample. Some of these instruments are miniaturized in nature and can be carried to the analysis site. However, most of them are not portable. In recent years, the interest in the fabrication of portable separation instrument has been continuously increasing. This chapter provides information on the requirements for miniaturization of the most commonly used instruments. Also, it reviews attempts to construct portable hyphenated instruments. Previous chapter in