At first a PD@His.GQD carrier, polymer modified histidine-functionalized graphene quantum dot, was synthesized to investigate the in vitro sunitinib (STB) delivery via luminescence spectrometer. The carrier’s synthesis, with average size of 34.0 nm, was proved by Fourier transform infrared (FTIR), Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS) analyses. In in vitro STB delivery investigation showed that for a healthy tissue, the STB was loaded at pH=7.2 and at 25 ᵒC with maximum loading efficiency percentage of 99% while it was released at pH=5.4 at 37 ᵒC with release percentage of 97%. In the sequel, the STB loaded carrier (STB.PD@His.GQD) was labeled with 67Ga (67Ga-STB.PD@His.GQD) to produce transparent radio-carrier for in vivo kidney cancerous mice imaging via the SPECT device. The radiochemical purity of the 67Ga-STB.PD@His.GQD was obtained as 95% by Radio Thin Layer Chromatography (RTLC) and High-Performance Liquid Chromatography (HPLC) analysis. These results confirmed that the synthesized PD@His.GQD is a stimuli-sensitive targeting carrier. All the cancerous mice body in vivo images at 10 and 20 hours 67Ga-STB.PD@His.GQD post-injection and its bio-distribution calculations showed its most accumulation in the kidney cancerous tissue. Also, the STB release kinetic was studied via Zero-order, First-order, Higuchi, and Korsmeyer-Peppas models and the release data were fitted with Korsmeyer-Peppas model.
