Anti-angiogenic (AA) drugs are proposed as novel agents for targeted therapies in hepatocellular carcinoma (HCC)[1]. One of these drugs is, Z-Sunitinib malate, a multi-targeted tyrosine kinase inhibitor (TKI) that is registered for the treatment of advanced clear cell renal cell carcinoma (RCC). Various methods have been used to determined sunitinib, including LC-MS/MS method, RP-HPLC method, UPLC-MS/MS, paper spray–MS method [2], Herein, we developed a sensitive, selective, rapid, facile, and especially green method based on the fluorescence quenching of N-GQDs as a highly fluorescent nitrogen-doped graphene quantum dots, a free metal quantum dot and facile biosensor, that obtained using a one-step pyrolysis of citric acid (CA) as a carbon source, and tris (hydroxymethyl) aminomethane (Tris-HMA) as a surface passivation agent, for determination of sunitinib malate in real samples . Prepared quantum dots were characterized by TEM , XRD and IR measurements. The influences initial pH, volume of N-GQDs and contact time have been researched in order to find the optimum quenching conditions. the calibration equation is A= 0.1909 C +0.4837 with a correlation coefficient of 0.999 (n=9).
