This paper has presented a novel strategy to carry out direct and sensitive determination of atropine in complex matrices based on the Co3O4-graphene modified carbon paste electrode. This novel nanostructure was characterized by different spectroscopic and electrochemical techniques including scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and electrochemical impedance spectroscopy. The fabricated electrochemical sensor showed good electrochemical response towards atropine. Under the optimized conditions, the calibration curve for atropine concentration was linear in the range from 0.1 to 3.2 μmol L−1 with the detection limit of 0.03 μmol L−1. In addition, the practical analytical performance of the sensor was examined by evaluating the selective detection of atropine in biological fluids and pharmaceutical samples with satisfied recovery. Therefore, the prepared sensor may hold great promise for fast, simple and sensitive detection and biomedical analysis of atropine in various real samples.
