In this paper, a method for the analysis of the chiral compounds enantiomeric composition is reported. The method is based on different interaction of the chiral compound enantiomers with chiral modified nanospheres. Light scattering properties of the synthesized nanospheres in the presence and the absence of chiral compounds was chosen as the detection signal. A significant change in the scattering intensity of the nanospheres takes place by the addition of the mixture of the enantiomers of a chiral compound. The results showed that d-(+)-tryptophan modified magnetite nanospheres were very sensitive to (R)-(−)-naproxen and d-(−)-α-phenylglycine and its corresponding RRS signal greatly enhanced in the presence of these enantiomers and l-(−)-tryptophan modified magnetite nanospheres was sensitive to (S)-(+)-naproxen and l-(+)-α-phenylglycine. The proposed method is feasible for sensitive analysis of the model chiral compound in pure form as low as ng L−1 concentration levels. The results showed that the synthesized nanospheres have excellent enantioselectivity behaviour in the case of investigated model chiral compounds. The proposed procedure can be used as an efficient method for the precise and accurate enantiomeric composition analysis of the chiral compounds.
